Extruder (liponizer), Membrane, Particle Distribution Analizer & Zeta-potential Analizer for Liposome Preparation
نویسندگان
چکیده
منابع مشابه
Functionalized liposome purification via Liposome Extruder Purification (LEP).
Liposome Extruder Purification (LEP) allows for the rapid purification of diverse liposome formulations using the same extrusion apparatus employed during liposome formation. The LEP process provides a means for purifying functionalized liposomes from non-conjugated drug or protein contaminants with >93% liposome recovery and >93% contaminant removal in a single step.
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Microand nano-particle charge is one of the main factors determining the physical stability of both emulsions and suspensions and can be quantified by measuring their so called “zeta potential”. When all the particles have a large either negative or positive zeta potential value, they will repel each other and, as a consequence, the suspension becomes stable. By contrast, whether the zeta poten...
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We developed a method, named GraDeR, which substantially improves the preparation of membrane protein complexes for structure determination by single-particle cryo-electron microscopy (cryo-EM). In GraDeR, glycerol gradient centrifugation is used for the mild removal of free detergent monomers and micelles from lauryl maltose-neopentyl glycol detergent stabilized membrane complexes, resulting i...
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Liposomes, sphere-shaped vesicles consisting of one or more phospholipid bilayers, were first described in the mid-60s. Today, they are a very useful reproduction, reagent, and tool in various scientific disciplines, including mathematics and theoretical physics, biophysics, chemistry, colloid science, biochemistry, and biology. Since then, liposomes have made their way to the market. Among sev...
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Various aspects of particle-stabilized emulsions (or so-called Pickering emulsions) have been extensively investigated during the last two decades, but the preparation of uniform Pickering emulsion droplets via a simple and scalable method has been sparingly realized. We report the preparation of uniform Pickering emulsions by Shirasu porous glass (SPG) membrane emulsification. The size of the ...
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ژورنال
عنوان ژورنال: membrane
سال: 1998
ISSN: 0385-1036,1884-6440
DOI: 10.5360/membrane.23.44